Cloning of inversion breakpoints in the Anopheles gambiae complex traces a transposable element at the inversion junction.

ABSTRACT: Anopheles arabiensis, one of the two most potent malaria vectors of the gambiae complex, is characterized by the presence of chromosomal paracentric inversions. Elucidation of the nature and the dynamics of these inversions is of paramount importance for the understanding of the population genetics and evolutionary biology of this mosquito and of the impact on malaria epidemiology. We report here the cloning of the breakpoints of the naturally occurring polymorphic inversion 2Rd' of A. arabiensis. A cDNA clone that cytologically mapped on the proximal breakpoint was the starting material for the isolation of a cosmid clone that spanned the breakpoint. Analysis of the surrounding sequences demonstrated that adjacent to the distal breakpoint lies a repetitive element that exhibits distinct d

SUBMITTER: Mathiopoulos KD 

PROVIDER: S-EPMC22850 | biostudies-literature | 1998 Oct

REPOSITORIES: biostudies-literature