Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue.

ABSTRACT:

Background

Investigating the expression of candidate genes in tissue samples usually involves either immunohistochemical labelling of formalin-fixed paraffin-embedded (FFPE) sections or immunofluorescence labelling of cryosections. Although both of these methods provide essential data, both have important limitations as research tools. Consequently, there is a demand in the research community to be able to perform routine, high quality immunofluorescence labelling of FFPE tissues.

Results

We present here a robust optimised method for high resolution immunofluorescence labelling of FFPE tissues, which involves the combination of antigen retrieval, indirect immunofluorescence and confocal laser scanning microscopy. We demonstrate the utility of this method with examples of imm

SUBMITTER: Robertson D 

PROVIDER: S-EPMC2288605 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature