Gene array and real time PCR analysis of the adrenal sensitivity to adrenocorticotropic hormone in pig.

ABSTRACT:

Background

Variability in hypothalamic-pituitary-adrenal (HPA) axis activity has been shown to be influenced by genetic factors and related to great metabolic differences such as obesity. The aim of this study was to investigate molecular bases of genetic variability of the adrenal sensitivity to ACTH, a major source of variability, in Meishan (MS) and Large White (LW) pigs, MS being reported to exhibit higher basal cortisol levels, response to ACTH and fatness than LW. A pig cDNA microarray was used to identify changes in gene expression in basal conditions and in response to ACTH stimulation.

Results

Genotype and/or ACTH affected the expression of 211 genes related to transcription, cell growth/maintenance, signal transduction, cell structure/adhesion/extra cellular matrix

SUBMITTER: Hazard D 

PROVIDER: S-EPMC2289818 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature