Functional maps of protein complexes from quantitative genetic interaction data.

ABSTRACT: Recently, a number of advanced screening technologies have allowed for the comprehensive quantification of aggravating and alleviating genetic interactions among gene pairs. In parallel, TAP-MS studies (tandem affinity purification followed by mass spectroscopy) have been successful at identifying physical protein interactions that can indicate proteins participating in the same molecular complex. Here, we propose a method for the joint learning of protein complexes and their functional relationships by integration of quantitative genetic interactions and TAP-MS data. Using 3 independent benchmark datasets, we demonstrate that this method is >50% more accurate at identifying functionally related protein pairs than previous approaches. Application to genes involved in yeast chromosome organ

SUBMITTER: Bandyopadhyay S 

PROVIDER: S-EPMC2289880 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature