Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.

ABSTRACT: Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not the

SUBMITTER: Nemethova M 

PROVIDER: S-EPMC2290848 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature