Global analysis of yeast endosomal transport identifies the vps55/68 sorting complex.

ABSTRACT: Endosomal transport is critical for cellular processes ranging from receptor down-regulation and retroviral budding to the immune response. A full understanding of endosome sorting requires a comprehensive picture of the multiprotein complexes that orchestrate vesicle formation and fusion. Here, we use unsupervised, large-scale phenotypic analysis and a novel computational approach for the global identification of endosomal transport factors. This technique effectively identifies components of known and novel protein assemblies. We report the characterization of a previously undescribed endosome sorting complex that contains two well-conserved proteins with four predicted membrane-spanning domains. Vps55p and Vps68p form a complex that acts with or downstream of ESCRT function to regulate

SUBMITTER: Schluter C 

PROVIDER: S-EPMC2291407 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature