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Experimental approach for assessing the outcome accuracy of antibody microarray experiments.


ABSTRACT: An experimental strategy for quality control of antibody microarray analyses is proposed. The method utilizes proteins that are prepared for regular antibody microarray experiments. There is no need to use exogenous positive or negative reference markers and no need to determine the absolute concentration of each individual protein in the sample. Validation experiments support the basic principle of the proposed approach. This method can be a useful tool for assessing the outcome accuracy of microarray experiments.

SUBMITTER: Gu Q 

PROVIDER: S-EPMC2291551 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Experimental approach for assessing the outcome accuracy of antibody microarray experiments.

Gu Qiang Q   Sivanandam Thamil Mani TM   Haymore Jessica J  

Journal of proteome research 20071002 11


An experimental strategy for quality control of antibody microarray analyses is proposed. The method utilizes proteins that are prepared for regular antibody microarray experiments. There is no need to use exogenous positive or negative reference markers and no need to determine the absolute concentration of each individual protein in the sample. Validation experiments support the basic principle of the proposed approach. This method can be a useful tool for assessing the outcome accuracy of mic  ...[more]

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