Identifying new PCR targets for pathogenic bacteria using top-down LC/MS protein discovery.

ABSTRACT: We have investigated the use of a top-down liquid chromatography/mass spectrometric (LC/MS) approach for the identification of specific protein biomarkers useful for differentiation of closely related strains of bacteria. The sequence information derived from the protein biomarker was then used to develop specific polymerase chain reaction primers useful for rapid identification of the strains. Shiga-toxigenic Escherichia coli (STEC) strains were used for this evaluation. The expressed protein profiles of two closely related serotype 0157:H7 strains, the predominant strain implicated in illness worldwide, and the nonpathogenic E. coli K-12 strain were compared with each other in an attempt to identify new protein markers that could be used to distinguish the 0157:H7 strains from each other

SUBMITTER: Williams TL 

PROVIDER: S-EPMC2291713 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature