Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.

ABSTRACT: We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study.

SUBMITTER: Jensen PH 

PROVIDER: S-EPMC2291755 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature