Hematopoietic chimerism monitoring based on STRs: quantitative platform performance on sequential samples.

ABSTRACT: Hematopoietic stem cell transplantation (HSCT) creates a donor-recipient cellular chimerism in the patient, which is quantitatively assayed from peripheral blood based on STR-DNA. Since chimerism values often vary across a patient's samples, it is important to determine to what extent this variability reflects technical aspects of platform performance. This issue is systematically assessed in the current study for the first time. Using the SGM Plus multiplex PCR kit and ABI platform, the longitudinal performance of STR markers was quantitatively evaluated in two chimeric models with true values, and in patient samples (n >500 marker loci). Computation of percent chimerism for each marker, and mean (sample) percent chimerism, standard deviation, and coefficient of variance was performed by

SUBMITTER: Kristt D 

PROVIDER: S-EPMC2291760 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature