In vivo cardiac imaging of adult zebrafish using high frequency ultrasound (45-75 MHz).

ABSTRACT: The zebrafish has emerged as an excellent genetic model organism for studies of cardiovascular development. Optical transparency and external development during embryogenesis allow for visual analysis in the early development. However, to understand the cardiovascular structures and functions beyond the early stage requires a high-resolution, real-time, noninvasive imaging alternative due to the opacity of adult zebrafish. In this research, we report the development of a high frequency ultrasonic system for adult zebrafish cardiac imaging, capable of 75 MHz B-mode imaging at a spatial resolution of 25 microm and 45 MHz pulsed-wave Doppler measurement. The system allows for real-time delineation of detailed cardiac structures, estimation of cardiac dimensions, as well as image-guided Dopple

SUBMITTER: Sun L 

PROVIDER: S-EPMC2292109 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature