Ex vivo induction of mRNA in human whole blood as a new platform of drug and dietary supplement development.

ABSTRACT:

Purpose

We introduced a new concept of ex vivo gene expression analysis (Mitsuhashi, Clin Chem 53:148-149, 2007), where drug action was simulated under physiological conditions. This model system was applied to study various fields of drug development.

Materials and methods

Heparinized human whole blood was incubated with drugs for less than 4h. The changes of specific mRNA were then quantified using the method we developed (Mitsuhashi, Tomozawa, Endo, and Shinagawa, Clin Chem 52:634-642, 2006).

Results

The mRNA quantitation method was used as a model system to study the following areas: (1) identification of respondents and non-respondents, (2) ex vivo compound screening, (3) determination of individually optimized doses, (4) drug-to-drug comparison, (5) assessment o

SUBMITTER: Mitsuhashi M 

PROVIDER: S-EPMC2292497 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature