In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water.

ABSTRACT: Pathogen detection tools with high reliability are needed for various applications, including food and water safety and clinical diagnostics. In this study, we designed and validated an in situ-synthesized biochip for detection of 12 microbial pathogens, including a suite of pathogens relevant to water safety. To enhance the reliability of presence/absence calls, probes were designed for multiple virulence and marker genes (VMGs) of each pathogen, and each VMG was targeted by an average of 17 probes. Hybridization of the biochip with amplicon mixtures demonstrated that 95% of the initially designed probes behaved as predicted in terms of positive/negative signals. The probes were further validated using DNA obtained from three different types of water samples and spiked with pathogen genom

SUBMITTER: Miller SM 

PROVIDER: S-EPMC2292592 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature