Inactivation and UV disinfection of murine norovirus with TiO2 under various environmental conditions.

ABSTRACT: We studied inactivation and UV disinfection of murine norovirus (MNV) as a surrogate for human norovirus. We investigated the effects of different surface characteristics, temperatures, and NaCl concentrations on MNV survival using both a plaque assay and a real-time TaqMan reverse transcription (RT)-PCR assay. MNV survived more than 40 days on diaper material, on gauze, and in a stool suspension. Compared to inactivation at lower temperatures (-20 and 4 degrees C), inactivation of MNV was greater at higher temperatures (18 and 30 degrees C). On the surface of both gauze and diaper material, there was a <2-log(10) reduction in the amount of infectious MNV in 40 days after incubation at both -20 and 4 degrees C, compared to a >5-log(10) reduction after incubation at 30 degrees C in 24 days.

SUBMITTER: Lee J 

PROVIDER: S-EPMC2292603 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature