Experimental evolution and genome sequencing reveal variation in levels of clonal interference in large populations of bacteriophage phiX174.

ABSTRACT:

Background

In large asexual populations where beneficial mutations may co-occur and recombination is absent, the fate of beneficial mutations can be significantly affected by competition (i.e., clonal interference). Theoretical models predict that clonal interference (CI) can slow adaptation, alter the distribution of fixed beneficial mutations, and affect disease progression by impacting within-host evolution of pathogens. While phenotypic data support that CI is a significant determinant of adaptive outcome, genetic data are needed to verify the patterns and to inform evolutionary models. We adapted replicate populations of the bacteriophage phiX174 under two levels of CaCl2 to create benign and harsh environments. Genomic sequences of multiple individuals from evolved population

SUBMITTER: Pepin KM 

PROVIDER: S-EPMC2292699 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature