Real-time PCR approach for detection of environmental sources of Campylobacter strains colonizing broiler flocks.

ABSTRACT: Reducing colonization of poultry flocks by Campylobacter spp. is a key strategy in the control and prevention human campylobacteriosis. Horizontal transmission of campylobacters, from in and around the farm, is the presumed route of flock colonization. However, the identification and prioritization of sources are confounded by the ubiquitous nature of these organisms in the environment, their poor rates of recovery by standard culture methods, and the need for cost-effective and timely methods for strain-specific comparison. A real-time PCR screening test for the strain-specific detection of campylobacters in environmental samples has been developed to address this issue. To enable this approach, fluorescently labeled PCR oligonucleotide probes suitable for a LightCycler-based assay were d

SUBMITTER: Ridley AM 

PROVIDER: S-EPMC2293161 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature