Effects of carbon dioxide on neurotoxin gene expression in nonproteolytic Clostridium botulinum Type E.

ABSTRACT: Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on the growth of and neurotoxin production by nonproteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulinum neurotoxin gene (cntE) and the formation of type E neurotoxin. The expression levels of cntE were similar in two strains, with relative expression peaking in the transition between exponential phase and stationary phase. In stationary phase, cntE mRNA expression declined rapidly. The cntE mRNA half-life was calculated to be approximately 9 minutes. Neurotoxin formation occurred in late exponen

SUBMITTER: Artin I 

PROVIDER: S-EPMC2293167 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature