The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse.

ABSTRACT: Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. The strain was constructed by serial genetic recombination steps, but the underlying sequence changes remained unverified. We report the complete genomic sequence of DH10B by using reads accumulated from the bovine sequencing project at Baylor College of Medicine and assembled with DNAStar's SeqMan genome assembler. The DH10B genome is largely colinear with that of the wild-type K-12 strain MG1655, although it is substantially more complex than previously appreciated, allowing DH10B biology to be further explored. The 226 mutated genes in DH10B relative to MG1655 are m

SUBMITTER: Durfee T 

PROVIDER: S-EPMC2293198 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature