A trap for in situ cultivation of filamentous actinobacteria.

ABSTRACT: The approach of growing microorganisms in situ, or in a simulated natural environment is appealing, and different versions of it have been described by several groups. The major difficulties with these approaches are that they are not selective for actinomycetes - a group of gram-positive bacteria well known as a rich source of antibiotics. In order to efficiently access actinomycetes, a trap for specifically capturing and cultivating these microorganisms in situ has been developed, based on the ability of these bacteria to form hyphae and penetrate solid environments. The trap is formed by two semi-permeable membranes (0.2-0.6 microm pore-size bottom membrane and 0.03 microm pore-size top membrane) glued to a plastic washer with sterile agar or gellan gum inside. The trap is placed on top

SUBMITTER: Gavrish E 

PROVIDER: S-EPMC2293972 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature