Screening for imprinted genes by allelic message display: identification of a paternally expressed gene impact on mouse chromosome 18.

ABSTRACT: A systematic screen termed the allelic message display (AMD) was developed for the hunting of imprinted genes. In AMD, differential display PCR is adopted to image allelic expression status of multiple polymorphic transcripts in two parental mouse strains, reciprocal F1 hybrids and pooled backcross progenies. From the displayed patterns, paternally and maternally expressed transcripts can be unequivocally identified. The effectiveness of AMD screening was clearly demonstrated by the identification of a paternally expressed gene Impact on mouse chromosome 18, the predicted product of which belongs to the YCR59c/yigZ hypothetical protein family composed of yeast and bacterial proteins with currently unknown function. In contrast with previous screening methods necessitating positional clonin

SUBMITTER: Hagiwara Y 

PROVIDER: S-EPMC23141 | biostudies-literature | 1997 Aug

REPOSITORIES: biostudies-literature