Identification and characterization of PhoP regulon members in Yersinia pestis biovar Microtus.

ABSTRACT:

Background

The transcription regulator PhoP has been shown to be important for Y. pestis survival in macrophages and under various in vitro stresses. However, the mechanism by which PhoP promotes bacterial intracellular survival is not fully understood. Our previous microarray analysis suggested that PhoP governed a wide set of cellular pathways in Y. pestis. A series of biochemical experiments were done herein to study members of the PhoP regulon of Y. pestis biovar Microtus.

Results

By using gel mobility shift assay and quantitative RT-PCR, a total of 30 putative transcription units were characterized as direct PhoP targets. The primer extension assay was further used to determine the transcription start sites of 18 PhoP-dependent promoters and to localize the -10 and -35

SUBMITTER: Li Y 

PROVIDER: S-EPMC2322996 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature