Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres.

ABSTRACT:

Background

Mammalian centromere formation is dependent on chromatin that contains centromere protein (CENP)-A, which is the centromere-specific histone H3 variant. Human neocentromeres have acquired CENP-A chromatin epigenetically in ectopic chromosomal locations on low-copy complex DNA. Neocentromeres permit detailed investigation of centromeric chromatin organization that is not possible in the highly repetitive alpha satellite DNA present at endogenous centromeres.

Results

We have examined the distribution of CENP-A, as well as two additional centromeric chromatin-associated proteins (CENP-C and CENP-H), across neocentromeric DNA using chromatin immunoprecipitation (ChIP) on CHIP assays on custom genomic microarrays at three different resolutions. Analysis of two neocentr

SUBMITTER: Alonso A 

PROVIDER: S-EPMC2323242 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature