Electrochemiluminescent/voltammetric toxicity screening sensor using enzyme-generated DNA damage.

ABSTRACT: Simultaneous optical and voltammetric detection of bioactivated genotoxicity is reported for the first time employing ultrathin films of DNA, model metabolic enzymes, and electrochemiluminescence (ECL) generating metallopolymer [Ru(bpy)2PVP10]2+ on pyrolytic graphite (PG) electrodes. Cytochrome P450cam and myoglobin were used as model monoxygenase enzymes to mimic in vivo processes. Sensor film growth and component amounts were monitored using a quartz crystal microbalance (QCM). Subsequent to the enzyme reaction, DNA damage in the sensor films was measured simultaneously using a simple apparatus combining a standard voltammetry cell coupled with an optical fiber and photomultiplier tube. The model enzyme reaction converted styrene to styrene oxide, which reacts with DNA nucleobases. ECL a

SUBMITTER: So M 

PROVIDER: S-EPMC2323599 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature