Distinct regulation of interleukin-17 in human T helper lymphocytes.

ABSTRACT:

Objective

Interleukin-17 (IL-17)-producing T helper cells have been proposed to represent a separate lineage of CD4+ cells, designated Th17 cells, which are regulated by the transcription factor retinoic acid-related orphan receptor gammat (RORgammat). However, despite advances in understanding murine Th17 differentiation, a systematic assessment of factors that promote the differentiation of naive human T cells to Th17 cells has not been reported. The present study was undertaken to assess the effects on naive human CD4+ T cells of cytokines known to promote murine Th17 cells.

Methods

Human naive and memory CD4+ T cells isolated from peripheral blood were activated and cultured with various cytokines. Cytokine production was measured by enzyme-linked immunosorbent assay and

SUBMITTER: Chen Z 

PROVIDER: S-EPMC2323677 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature