Rationally selected basis proteins: a new approach to selecting proteins for spectroscopic secondary structure analysis.

ABSTRACT: Protein basis sets have been extensively used as reference data for the determination of protein structure with optical methods such as circular dichroism and infrared spectroscopies. We have taken a new approach to basis protein selection by utilizing three crystal structure classification databases: CATH, SCOP, and PDB_SELECT. Through the use of the information available in these and other online resources, we identified 115 commercially available proteins as potential basis set candidates. By carefully screening the quality of the crystal structures and commercial protein preparations, we obtained a final set of 50 rationally selected proteins (RaSP50) that has been optimized for use in spectroscopic protein structure determination studies. These proteins span the full range of known pr

SUBMITTER: Oberg KA 

PROVIDER: S-EPMC2323998 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature