Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
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ABSTRACT:
SUBMITTER: Schulte R
PROVIDER: S-EPMC2329591 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Publications
Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
Schulte Roberta R Talamas Jessica J Doucet Christine C Hetzer Martin W MW
PloS one 20080430 4
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a ...[more]
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