Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.

ABSTRACT: We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.

SUBMITTER: Schulte R 

PROVIDER: S-EPMC2329591 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature