Molecular analysis reveals heterogeneity of mouse mammary tumors conditionally mutant for Brca1.

ABSTRACT:

Background

Development of therapies for patients with BRCA1 mutations has been hampered by lack of readily available in vitro and in vivo models. We recently showed that transplantation of transgenic mammary tumors as cell suspensions into naïve recipients generates reproducible tumors with remarkable stability of gene expression profile. We examined the expression profiles of original and serially transplanted mammary tumors from Brca1 deficient mice, and tumor derived cell lines to validate their use for preclinical testing and studies of tumor biology.

Methods

Original tumors, serially transplanted and multiple cell lines derived from Brca1 mammary tumors were characterized by morphology, gene and protein expression, and cell surface markers.

Results

Gene expressio

SUBMITTER: Wright MH 

PROVIDER: S-EPMC2329667 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature