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Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus.

ABSTRACT: The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K(2)) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222(1), with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 A. The crystal diffracted to a resolution of 2.2 A. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.

SUBMITTER: Kanaujia SP 

PROVIDER: S-EPMC2330136 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature

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Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus.

Kanaujia Shankar Prasad SP   Ranjani Chellamuthu Vasuki CV   Jeyakanthan Jeyaraman J   Baba Seiki S   Kuroishi Chizu C   Ebihara Akio A   Shinkai Akeo A   Kuramitsu Seiki S   Shiro Yoshitsugu Y   Sekar Kanagaraj K   Yokoyama Shigeyuki S  

Acta crystallographica. Section F, Structural biology and crystallization communications 20070117 Pt 2

The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K(2)) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222(1), with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 A. The crystal diffracted to a resolution of 2.2 A. Pr  ...[more]

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