Can subtle changes in gene expression be consistently detected with different microarray platforms?

ABSTRACT:

Background

The comparability of gene expression data generated with different microarray platforms is still a matter of concern. Here we address the performance and the overlap in the detection of differentially expressed genes for five different microarray platforms in a challenging biological context where differences in gene expression are few and subtle.

Results

Gene expression profiles in the hippocampus of five wild-type and five transgenic deltaC-doublecortin-like kinase mice were evaluated with five microarray platforms: Applied Biosystems, Affymetrix, Agilent, Illumina, LGTC home-spotted arrays. Using a fixed false discovery rate of 10% we detected surprising differences between the number of differentially expressed genes per platform. Four genes were selected by A

SUBMITTER: Pedotti P 

PROVIDER: S-EPMC2335120 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature