Using quality scores and longer reads improves accuracy of Solexa read mapping.

ABSTRACT:

Background

Second-generation sequencing has the potential to revolutionize genomics and impact all areas of biomedical science. New technologies will make re-sequencing widely available for such applications as identifying genome variations or interrogating the oligonucleotide content of a large sample (e.g. ChIP-sequencing). The increase in speed, sensitivity and availability of sequencing technology brings demand for advances in computational technology to perform associated analysis tasks. The Solexa/Illumina 1G sequencer can produce tens of millions of reads, ranging in length from approximately 25-50 nt, in a single experiment. Accurately mapping the reads back to a reference genome is a critical task in almost all applications. Two sources of information that are often ignore

SUBMITTER: Smith AD 

PROVIDER: S-EPMC2335322 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature