Rapid comparative genomic analysis for clinical microbiology: the Francisella tularensis paradigm.

ABSTRACT: It is critical to avoid delays in detecting strain manipulations, such as the addition/deletion of a gene or modification of genes for increased virulence or antibiotic resistance, using genome analysis during an epidemic outbreak or a bioterrorist attack. Our objective was to evaluate the efficiency of genome analysis in such an emergency context by using contigs produced by pyrosequencing without time-consuming finishing processes and comparing them to available genomes for the same species. For this purpose, we analyzed a clinical isolate of Francisella tularensis subspecies holarctica (strain URFT1), a potential biological weapon, and compared the data obtained with available genomic sequences of other strains. The technique provided 1,800,530 bp of assembled sequences, resulting in 48

SUBMITTER: La Scola B 

PROVIDER: S-EPMC2336804 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature