Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.

ABSTRACT: The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.

SUBMITTER: Nishida K 

PROVIDER: S-EPMC2344109 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature