Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereus ATCC 14579.

ABSTRACT:

Background

The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcriptome analysis of the wild-type and a ccpA-deletion strain.

Results

Comparative analysis revealed the growth performance and glucose consumption rates to be lower in the B. cereus ATCC 14579 ccpA deletion strain than in the wild-type. In exponentially grown cells, the expression of glycolytic genes, including a non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase that mediates conversion of D-glyceraldehyde 3-phosphate to 3-phospho-D-glycerate in one singl

SUBMITTER: van der Voort M 

PROVIDER: S-EPMC2358912 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature