Inflammasome mRNA expression in human monocytes during early septic shock.

ABSTRACT:

Rationale

Monocytes are central to the initiation of the inflammatory response in sepsis, with caspase-1 activation playing a key role. Monocyte deactivation during sepsis has been linked to poor outcomes.

Objectives

Given the importance of caspase-1 in the immune response, we investigated whether monocytes from patients early in septic shock demonstrate alterations in mRNAs for caspase-1-related molecules.

Methods

Patients with septic shock (n = 26; age >18 years), critically ill intensive care unit patients (n = 20), and healthy volunteers (n = 22) were enrolled in a prospective cohort study in a university intensive care unit. Demographic, biological, physiologic, and plasma cytokine measurements were obtained. Monocytes were assayed for ex vivo tumor necrosis fact

SUBMITTER: Fahy RJ 

PROVIDER: S-EPMC2361424 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature