Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

ABSTRACT:

Background

The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencing are being used for examination, but, beside methodological limitations, an increasing number of recently tested strains could not be assigned to any established genotype.

Results

Comprehensive analysis of all available ompA gene sequences has revealed a remarkable genetic diversity within the species C. psittaci, which is only partially covered by the present genotyping scheme. We suggest adjustments and extensions to the present scheme, which include the int

SUBMITTER: Sachse K 

PROVIDER: S-EPMC2362127 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature