Selection and characterisation of a phage-displayed human antibody (Fab) reactive to the lung resistance-related major vault protein.

ABSTRACT: The major vault protein is the main component on multimeric vault particles, that are likely to play an essential role in normal cell physiology and to be associated with multidrug resistance of tumour cells. In order to unravel the function of vaults and their putative contribution to multidrug resistance, specific antibodies are invaluable tools. Until now, only conventional major vault protein-reactive murine monoclonal antibodies have been generated, that are most suitable for immunohistochemical analyses. The phage display method allows for selection of human antibody fragments with potential use in clinical applications. Furthermore, cDNA sequences encoding selected antibody fragments are readily identified, facilitating various molecular targeting approaches. In order to obtain such

SUBMITTER: Scheffer GL 

PROVIDER: S-EPMC2364164 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature