JunD activates transcription of the human ferritin H gene through an antioxidant response element during oxidative stress.

ABSTRACT: Ferritin is the major intracellular iron storage protein that sequesters excess free iron to minimize generation of iron-catalysed reactive oxygen species. We previously demonstrated that expression of ferritin heavy chain (ferritin H) was induced by pro-oxidants, which is a part of cellular antioxidant response to protect cells from oxidative damage. In this study, we have identified that the antioxidant/electrophile response element (ARE) located 4.5 kb upstream to the human ferritin H transcription initiation site is responsible for the oxidant response. The human ferritin H ARE comprises two copies of bidirectional AP1 motifs. Mutations in each AP1 motif significantly impaired protein binding and the function of the ARE, indicating that both of the AP1 motifs are required for pro-oxida

SUBMITTER: Tsuji Y 

PROVIDER: S-EPMC2365508 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature