Dual roles for Prox1 in the regulation of the chicken betaB1-crystallin promoter.

ABSTRACT:

Purpose

Lens fiber cell differentiation is marked by the onset of betaB1-crystallin expression and is controlled by the cooperative action of a set of transcription factors including Prox1, an atypical homeodomain protein. Previously, the authors reported that Prox1 directly interacts with the OL2 element found in the chicken betaB1-crystallin basal promoter to activate the expression of this gene. Here they mapped the location of activating and repressing sequences of the full-length chicken betaB1-crystallin promoter (-432/+30) in lens epithelial cells, annular pad cells, and intact lens and characterized Prox1-binding sites found in this region.

Methods

Transfection analysis and transgenic mice were used to characterize upstream regions of the chicken betaB1-crystallin ge

SUBMITTER: Chen X 

PROVIDER: S-EPMC2366088 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature