Semi-automated microseeding of nanolitre crystallization experiments.

ABSTRACT: A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.

SUBMITTER: Walter TS 

PROVIDER: S-EPMC2373990 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature