Leucine 41 is a gate for water entry in the reduction of Clostridium pasteurianum rubredoxin.

ABSTRACT: Biological electron transfer is an efficient process even though the distances between the redox moieties are often quite large. It is therefore of great interest to gain an understanding of the physical basis of the rates and driving forces of these reactions. The structural relaxation of the protein that occurs upon change in redox state gives rise to the reorganizational energy, which is important in the rates and the driving forces of the proteins involved. To determine the structural relaxation in a redox protein, we have developed methods to hold a redox protein in its final oxidation state during crystallization while maintaining the same pH and salt conditions of the crystallization of the protein in its initial oxidation state. Based on 1.5 A resolution crystal structures and mole

SUBMITTER: Min T 

PROVIDER: S-EPMC2374124 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature