Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.

ABSTRACT: Proteins in the small subunit of the mammalian mitochondrial ribosome were separated by two-dimensional polyacrylamide gel electrophoresis. Four individual proteins were subjected to in-gel Endoprotease Lys-C digestion. The sequences of selected proteolytic peptides were obtained by electrospray tandem mass spectrometry. Peptide sequences obtained from in-gel digestion of individual spots were used to screen human, mouse, and rat expressed sequence tag databases, and complete consensus cDNAs for these species were deduced in silico. The corresponding protein sequences were characterized by comparison to known ribosomal proteins in protein databases. Four different classes of mammalian mitochondrial small subunit ribosomal proteins were identified. Only two of these proteins have significan

SUBMITTER: Koc EC 

PROVIDER: S-EPMC2374141 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature