Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an in vitro study.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is a chronic, inflammatory and systemic autoimmune disease that leads to progressive cartilage destruction. Advances in the treatment of RA-related destruction of cartilage require profound insights into the molecular mechanisms involved in cartilage degradation. Until now, comprehensive data about the molecular RA-related dysfunction of chondrocytes have been limited. Hence, the objective of this study was to establish a standardized in vitro model to profile the key regulatory molecules of RA-related destruction of cartilage that are expressed by human chondrocytes.

Methods

Human chondrocytes were cultured three-dimensionally for 14 days in alginate beads and subsequently stimulated for 48 hours with supernatants from SV40 T-antigen imm

SUBMITTER: Andreas K 

PROVIDER: S-EPMC2374452 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature