Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity.

ABSTRACT:

Background

Recently, several populations of postnatal stem cells, such as multipotent adult progenitor cells (MAPCs), have been described that have broader differentiation ability than classical adult stem cells. Here we compare the transcriptome of pluripotent embryonic stem cells (ESCs), MAPCs, and lineage-restricted mesenchymal stem cells (MSCs) to determine their relationship.

Results

Applying principal component analysis, non-negative matrix factorization and k-means clustering algorithms to the gene-expression data, we identified a unique gene-expression profile for MAPCs. Apart from the ESC-specific transcription factor Oct4 and other ESC transcripts, some of them associated with maintaining ESC pluripotency, MAPCs also express transcripts characteristic of early endo

SUBMITTER: Ulloa-Montoya F 

PROVIDER: S-EPMC2374994 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature