Using macro-arrays to study routes of infection of Helicobacter pylori in three families.

ABSTRACT:

Background

Analysis of the evolutionary dynamics of Helicobacter pylori allowed tracing the spread of infection through populations on different continents but transmission pathways between individual humans have not been clearly described.

Materials and methods

To investigate person-to-person transmission, we studied three families each including one child with persistence of symptoms after antibiotic treatment. Ten isolates from the antrum and corpus of stomach of each family member were analyzed both by sequencing of two housekeeping genes and macroarray tests.

Results

A total of 134 (8.4%) out of the 1590 coding sequences (CDSs) tested, including cag PAI and insertion sequences, were present in some but not all isolates (and are therefore defined as variable CDSs)

SUBMITTER: Raymond J 

PROVIDER: S-EPMC2375058 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature