Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression.

ABSTRACT: Survivin is a novel member of the inhibitor of apoptosis family and determines the susceptibility of tumour cells to pro-apoptotic stimuli. Recently, we identified two novel alternative splice variants of survivin, differing in their anti-apoptotic properties: whereas the anti-apoptotic potential of survivin-DeltaEx3 is preserved, survivin-2B has lost its anti-apoptotic potential and may act as a naturally occurring antagonist of survivin. Because the in vivo expression of these alternative splice variants has not been explored so far, we analysed gastric carcinomas of different histological subtypes, grades and stages. Since no antibodies are currently available to determine the novel splice variants, quantitative reverse transcriptase polymerase chain reaction was performed, using RNA sa

SUBMITTER: Krieg A 

PROVIDER: S-EPMC2375298 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature