High quality metabolomic data for Chlamydomonas reinhardtii.

ABSTRACT: The green eukaryote alga Chlamydomonas reinhardtii is a unicellular model to study control of metabolism in a photosynthetic organism. We here present method improvements for metabolite profiling based on GC-TOF mass spectrometry focusing on three parameters: quenching and cell disruption, extract solvent composition and metabolite annotation. These improvements facilitate using smaller cell numbers and hence, smaller culture volumes which enable faster and more precise sampling techniques that eventually lead to a higher number of samples that can be processed, e.g. for time course experiments. Quenching of metabolism was achieved by mixing 1 ml of culture to 1 ml of -70 degrees C cold 70% methanol. After centrifugation, cells were lyophilized and disrupted by milling using 2-6E6 lyophili

SUBMITTER: Lee DY 

PROVIDER: S-EPMC2377246 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature