Metabolic pathways of the wheat (Triticum aestivum) endosperm amyloplast revealed by proteomics.

ABSTRACT:

Background

By definition, amyloplasts are plastids specialized for starch production. However, a proteomic study of amyloplasts isolated from wheat (Triticum aestivum Butte 86) endosperm at 10 days after anthesis (DPA) detected enzymes from many other metabolic and biosynthetic pathways. To better understand the role of amyloplasts in food production, the data from that study were evaluated in detail and an amyloplast metabolic map was outlined.

Results

Analysis of 288 proteins detected in an amyloplast preparation predicted that 178 were amyloplast proteins. Criteria included homology with known plastid proteins, prediction of a plastid transit peptide for the wheat gene product or a close homolog, known plastid location of the pathway, and predicted plastid location for ot

SUBMITTER: Dupont FM 

PROVIDER: S-EPMC2383896 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature