PCR-DGGE assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis.

ABSTRACT:

Background

Infectious mastitis is a common condition during lactation and in fact, represents one of the main causes leading to a precocious weaning. The number of studies dealing with lactational mastitis is low and, up to now, the etiological diagnosis is frequently made on the basis of unspecific clinical signs. The aim of this study was to investigate the microbial diversity of breast milk in 20 women with lactational mastitis employing culture-dependent and culture-independent (PCR-DGGE) approaches.

Methods

Breast milk samples were cultured in different media to investigate the presence of bacteria and/or yeasts, and a total of 149 representative isolates were identified to the species level by 16S rRNA gene PCR sequencing. The microorganisms recovered were compared wit

SUBMITTER: Delgado S 

PROVIDER: S-EPMC2383900 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature