Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens.

ABSTRACT:

Background

Diplomonads are common free-living inhabitants of anoxic aquatic environments and are also found as intestinal commensals or parasites of a wide variety of animals. Spironucleus vortens is a putatively commensal diplomonad of angelfish that grows to high cell densities in axenic culture. Genomic sequencing of S. vortens is in progress, yet little information is available regarding molecular and cellular aspects of S. vortens biology beyond descriptive ultrastructural studies. To facilitate the development of S. vortens as an additional diplomonad experimental model, we have constructed and stably transformed an episomal plasmid containing an enhanced green fluorescent protein (GFP) tag, an AU1 epitope tag, and a tandem affinity purification (TAP) tag. This construct also

SUBMITTER: Dawson SC 

PROVIDER: S-EPMC2386477 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature