Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici.

ABSTRACT:

Background

Puccinia striiformis f. sp. tritici is an obligate biotrophic pathogen that causes leaf stripe rust on wheat. Although it is critical to understand molecular mechanisms of pathogenesis in the wheat stripe rust fungus for developing novel disease management strategies, little is known about its genome and gene functions due to difficulties in molecular studies with this important pathogen. To identify genes expressed during early infection stages, in this study we constructed a cDNA library with RNA isolated from urediniospores of P. striiformis f. sp. tritici germinated for 10 h.

Results

A total of 4798 ESTs were sequenced from the germinated urediniospore library and assembled into 315 contigs and 803 singletons. About 23.9% and 13.3% of the resulting 1118 uniseq

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC2386484 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature